Calculation of deviation
Figure 4 shows the flow chart for the calibration work. The flow on the right is used for measurement and the flow on the left is used to calibrate the calculation of the baseline. The value of TCB can be calculated based on the size of the DBA, the size of the probe probe, and the mounting error of the double-ball sample obtained by measurement on the calibrated gear measuring instrument. The radius of the base circle is debugged by the operator of the calibration work, and then the peak point A, the valley bottom V and the peak point B of the TCB curve and the MCB curve are respectively determined.
Figure 4 Logic flow chart of the involute detector using a spherical template
In order to compensate for the TCB and the MCB, the TCB curve is moved in parallel along the X-axis direction or the Y-axis direction until the root mean square value of the obtained (MCB value - TCB value after parallel movement) is the smallest. When fitting the curve, the method of determining the x (expansion angle) range is generally such that the y value corresponding to the x maximum and minimum values ​​should be equal to the valley bottom depth of the curve (see Figure 2).
In order to simplify the somewhat troublesome curve fitting process, the displacement amount of the x-axis (on the expansion angle) can be successfully determined by satisfying the following conditions: the theoretical value of the peak point A, the valley V, and the peak point B of the TCB curve are added. The sum of the sums is equal to the sum of the MCBs because this sum is almost unaffected by the parameter changes. The effect of the y-axis displacement error during curve fitting can be eliminated by simply subtracting a constant value. This will give you the DCE curve for this measurement.
By changing the parameters, we can artificially draw different TCB curves. For the case where there is a deviation in the amplification ratio of the detector, the TCB curve can be obtained by multiplying the output value of the hump curve by a multiple. After obtaining the DCE curve using this modified TCB curve, we can roughly determine the possible cause of the inaccuracy of the calibration instrument: when a certain set of parameters is found to make the DCE curve very flat, this set of parameters prompts. The instrument may be inaccurate. Through this calibration procedure, the position error of the probe probe on the template, the magnification ratio deviation and the adjustment error of the base circle radius can usually be well determined.
Previous page next page
Universal Pipette Tips, which are disposable consumables, are specially designed to reduce the residual adsorption of samples during the pipetting process. They can be used in any molecular biology and genetic research applications. Yongyue Medical's universal Pipette tips can be used in different applications. brand of pipettes. Sterile filter pipette tips is an upgraded version of universal pipette tips, that is, a filter element is added to the upper end of universal pipette tips, and the pore size is optimized to ensure the barrier effect and good ventilation.
Universal Filter Tips,Filtered Tips,Racked Pipette Tips,Micropipette Tips,Sterile Filter Pipette Tips
Yong Yue Medical Technology(Kunshan) Co.,Ltd , https://www.yonyuemedicalcare.com